EF-18 Agar is used for the selective and differential isolation of Salmonella, using the ISO-GRID® and/or NEO-GRID™ Membrane Filtration System. EF-18 Agar is not intended for use in the diagnosis of disease or other conditions in humans.

EF-18 Agar was developed for the primary selective isolation of presumptive Salmonella spp. from enrichment broth using the ISO-GRID System. This medium suppresses growth of Gram-positive bacteria and certain Gram-negative bacteria, while enhancing growth of Salmonella spp. EF-18 Agar aids in differentiating Salmonella spp. from other Enterobacteriaceae based on lysine decarboxylase and sucrose fermentation reactions. EF-18 Agar is incubated at 42°C, providing additional selectivity against certain Gram-negative bacteria.

EF-18 Agar is recommended for the primary differential isolation and detection of Salmonella spp. from all foods using the ISO-GRID and /or NEO-GRID Membrane Filtration System.


Formula Liter
Enzymatic Digest of Animal Tissue 5 g
Yeast Extract 3 g
L-Lysine 10 g
Dextrose 2.5 g
Sucrose 15 g
Magnesium Sulfate 1.5 g
Bile Salts 1.5 g
Sulfapyridine 0.3 g
Bromthymol Blue 0.03 g
Novobiocin 0.015 g
Agar 15 g

Final pH: 6.8 ± 0.1 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 54 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.

Order Details

Product # Product Description
6901A EF-18 Agar, 500 g

Reference Material

6901 - EF-18 Agar, Product Information Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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