EF-18 Agar is used for the selective and differential isolation of Salmonella, using the ISO-GRID® and/or NEO-GRID™ Membrane Filtration System. EF-18 Agar is not intended for use in the diagnosis of disease or other conditions in humans.
EF-18 Agar was developed for the primary selective isolation of presumptive Salmonella spp. from enrichment broth using the ISO-GRID System. This medium suppresses growth of Gram-positive bacteria and certain Gram-negative bacteria, while enhancing growth of Salmonella spp. EF-18 Agar aids in differentiating Salmonella spp. from other Enterobacteriaceae based on lysine decarboxylase and sucrose fermentation reactions. EF-18 Agar is incubated at 42°C, providing additional selectivity against certain Gram-negative bacteria.
EF-18 Agar is recommended for the primary differential isolation and detection of Salmonella spp. from all foods using the ISO-GRID and /or NEO-GRID Membrane Filtration System.
|Enzymatic Digest of Animal Tissue||5 g|
|Yeast Extract||3 g|
|Magnesium Sulfate||1.5 g|
|Bile Salts||1.5 g|
|Bromthymol Blue||0.03 g|
Final pH: 6.8 ± 0.1 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
- Suspend 54 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- DO NOT AUTOCLAVE.
|Product #||Product Description|
|6901A||EF-18 Agar, 500 g|
6901 - EF-18 Agar, Product Information Sheet [English]
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.