Product number: 7361

EC Medium with MUG is used for the fluorogenic detection of Escherichia coli in a laboratory setting. EC Medium with MUG is not intended for use in the diagnosis of disease or other conditions in humans.

EC Medium was developed by Hajna and Perry in an effort to improve the methods for the detection of the coliform group and E. coli. This medium consists of a buffered lactose broth with the addition of 0.15% Bile Salts Mixture. Growth of spore-forming bacteria is inhibited by the bile salts, while growth of E. coli is enhanced by its presence. EC Medium with MUG is the same formula as EC Medium, with the addition of 4-methylumbelliferyl-β-D-glucuronide. Feng and Hartman developed a rapid assay for E. coli by incorporating 4-methylumbelliferyl-β-D-glucuronide (MUG) into Lauryl Tryptose Broth at a final concentration of 100 µg/mL. Moburg determined the amount of MUG could be reduced to a final concentration of 50 µg/mL without adversely affecting results.

EC Medium with MUG is prepared according to the formula specified by US EPA and methods for water and food testing.

 

Formula Liter
Tryptose 20 g
Lactose 5 g
Bile Salts Mixture 1.5 g
Dipotassium Phosphate 4 g
Monopotassium Phosphate 1.5 g
Sodium Chloride 5 g
4-Methylumbelliferyl-β-D-Glucuronide 0.05 g

Final pH: 6.9 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 37 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Distribute into tubes containing inverted Durham tubes.
  4. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7361A EC Medium with MUG, 500 g
7361T EC Medium with MUG, 5 kg
7361C EC Medium with MUG, 10 kg

Reference Material

7361 - EC Medium with MUG, Product Information Sheet [English] [Portuguese]
7361 - EC Medium with MUG, Safety Data Sheet [English] [Portuguese]

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