Product number: 7186

Deoxycholate Citrate Agar is used for the isolation and differentiation of Gram-negative enteric bacilli in a laboratory setting. Deoxycholate Citrate Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Deoxycholate Citrate Agar is a modification of Deoxycholate Agar formulated by Leifson. His original formula, Deoxycholate Agar, was used for isolation of intestinal pathogens and enumeration of intestinal pathogens in milk and water. Deoxycholate Agar was an improvement over other media because citrates and sodium deoxycholate worked well as inhibitors.

Leifson modified the original medium by increasing the concentration of Sodium Citrate and Sodium Deoxycholate for improved recovery of Salmonella spp. and Shigella spp. Deoxycholate Citrate Agar effectively isolates intestinal pathogens by inhibiting coliforms and many Proteus spp. This medium is used to screen Salmonella spp. and Shigella spp. from samples.


Formula Liter
Pork Infusion Solids 10 g
Enzymatic Digest of Animal Tissue 10 g
Lactose 10 g
Sodium Citrate 20 g
Ferric Citrate 1 g
Sodium Deoxycholate 5 g
Neutral Red 0.02 g
Agar 17 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 73 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.

Order Details

Product # Product Description
7186A Deoxycholate Citrate Agar, 500 g

Reference Material

7186 - Deoxycholate Citrate Agar, Product Information Sheet [English]

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