Product number: 7130

Deoxycholate Agar is used for the isolation and differentiation of Gram-negative enteric bacilli in a laboratory setting. Deoxycholate Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Deoxycholate Agar was described first by Leifson for isolation of intestinal pathogens and the enumeration of intestinal pathogens in milk and water. Deoxycholate Agar was an improvement over other media because of the chemicals, citrates and sodium deoxycholate worked well as inhibitors. This medium is used to screen for Salmonella spp. and Shigella spp.

 

Formula Liter
Enzymatic Digest of Casein 5 g
Enzymatic Digest of Animal Tissue 5 g
Lactose 10 g
Sodium Deoxycholate 1 g
Sodium Chloride 5 g
Dipotassium Phosphate 2 g
Ferric Citrate 1 g
Sodium Citrate 1 g
Neutral Red 0.03 g
Agar 16 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 46 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. DO NOT AUTOCLAVE.

Order Details

Product # Product Description
7130A Deoxycholate Agar, 500 g

Reference Material

7130 - Deoxycholate Agar, Product Information Sheet [English]

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