Product number: 7126
Columbia CNA Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Columbia CNA Agar is not intended for use in the diagnosis of disease or other conditions in humans.
Ellner et al. described Columbia CNA Agar as a variation of Columbia Blood Agar Base that is selective for Gram-positive cocci. Colistin and Nalidixic Acid are added to the formula, selecting for Gram-positive organisms and fungi by suppressing Gram-negative bacteria.
|Enzymatic Digest of Casein||5 g|
|Enzymatic Digest of Animal Tissue||8 g|
|Yeast Enriched Peptone||10 g|
|Corn Starch||1 g|
|Sodium Chloride||5 g|
|Nalidixic Acid||0.01 g|
Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
- Suspend 43 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes. Do not overheat medium.
- Prepare 5% blood agar by aseptically adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45 – 50°C.
|Product #||Product Description|
|7126A||Columbia CNA Agar, 500 g|
|7126T||Columbia CNA Agar, 5 kg|
|7126C||Columbia CNA Agar, 10 kg|