Product number: 7385
Clostridium difficile Agar is used with blood, Cycloserine, and Cefoxitin for the isolation of Clostridium difficile in a laboratory setting. Clostridium difficile Agar is not intended for use in the diagnosis of disease or other conditions in humans.
Clostridium difficile has been isolated from diverse natural habitats, including soil, hay, sand, dung from various large mammals, and from dog, cat, and rodent feces.
In 1979, George et al. developed a medium called CCFA (Cycloserine-Cefoxitin-Fructose agar), based on the Egg Yolk Agar formula of McClung and Toabe with fructose replacing glucose. Clostridium difficile Agar is a modification of the original CCFA formulation.
|Enzymatic Digest of Casein||15 g|
|Enzymatic Digest of Animal Tissue||15 g|
|Pork Brain Heart Infusion Solids||10 g|
|Disodium Phosphate||5 g|
|Monopotassium Phosphate||1 g|
|Magnesium Sulfate||0.1 g|
|Sodium Chloride||2 g|
Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Horse Blood, 7%
Cycloserine, 0.5 g/L
Cefoxitin, 0.016 g/L
- Suspend 69 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
- Cool to 45 – 50°C and aseptically add 7% horse blood, Cycloserine (0.5 g/L) and Cefoxitin (0.016 g/L).
|Product #||Product Description|
|7385A||Clostridium difficile Agar, 500 g|
|7385T||Clostridium difficile Agar, 5 kg|
|7385C||Clostridium difficile Agar, 10 kg|