Product number: 7123
Casman Medium Base is used with blood for the isolation of Haemophilus influenzae, Neisseria gonorrhoeae and other fastidious microorganisms in a laboratory setting. Casman Medium Base is not intended for use in the diagnosis of disease or other conditions in humans.
In 1947, Casman described an infusion-free medium enriched with 5% blood for fastidious microorganisms incubated anaerobically. This medium replaced formulas containing fresh meat infusion and heated blood. Casman adjusted the formula after experiments revealed nicotinamide disrupted the action of a blood enzyme that inactivates V factor (NAD). The concentration of nicotinamide was lowered to support growth of Neisseria spp.
|Enzymatic Digest of Casein||5 g|
|Enzymatic Digest of Animal Tissue||5 g|
|Yeast Enriched Peptone||10 g|
|Beef Extract||3 g|
|p-Aminobenzoic Acid||0.05 g|
|Corn Starch||1 g|
|Sodium Chloride||5 g|
Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
- Suspend 43 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
- After cooling medium to 50°C, aseptically add 5% sterile blood and 0.15% sterile water-lysed blood solution (one part blood to three parts water).
|Product #||Product Description|
|7123A||Casman Medium Base, 500 g|
|7123T||Casman Medium Base, 5 kg|
|7123C||Casman Medium Base, 10 kg|