Product number: 7119

Brilliant Green Bile Broth 2% is used for the detection of coliform bacteria in water, food, and dairy products in a laboratory setting. Brilliant Green Bile Broth 2% is not intended for use in the diagnosis of disease or other conditions in humans.

The coliform group of bacteria includes aerobic and facultative anaerobic, Gram-negative, non-sporeforming bacilli that ferment lactose and form acid and gas at 35°C within 48 hours. Members of the Enterobacteriacae comprise the majority of this group, but organisms such as Aeromonas spp. may also be included. Procedures to detect and confirm coliforms are used in testing water, foods, dairy products and other materials. Brilliant Green Bile Broth 2% is used to confirm a positive presumptive test result.

Brilliant Green Bile Broth 2% is also referred to as Brilliant Green Bile Broth, Brilliant Green Lactose Broth, Brilliant Green Lactose Bile Broth and Brilliant Green Lactose Bile Broth, 2%.


Formula Liter
Enzymatic Digest of Gelatin 10 g
Lactose 10 g
Oxbile 20 g
Brilliant Green 0.0133 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 40 g of the medium in one liter of purified water until evenly dispersed.
  2. Heat with frequent agitation to completely dissolve the medium.
  3. Distribute into fermentation tubes.
  4. Autoclave at 121°C for no longer than 15 minutes. To avoid entrapment of bubbles in the fermentation tubes, allow the autoclave to cool at least to 75°C before opening.

Order Details

Product # Product Description
7119A Brilliant Green Bile Broth 2%, 500 g
7119T Brilliant Green Bile Broth 2%, 5 kg
7119C Brilliant Green Bile Broth 2%, 10 kg

Reference Material

7119 - Brilliant Green Bile Broth 2%, Product Information Sheet [English] [Portuguese]
7119 - Brilliant Green Bile Broth 2%, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]

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