Product number: 7115
Brain-Heart Infusion Agar is used for the cultivation of a wide variety of fastidious organisms in a laboratory setting. Brain-Heart Infusion Agar is not intended for use in the diagnosis of disease or other conditions in humans.
Rosenow prepared a rich medium for culturing streptococci by combining dextrose broth and brain tissue. Hayden modified the original formula while working with dental pathogens. The current formula is a modification of Rosenow and Hayden, using dehydrated infusions of pork brain and pork heart tissue.
Brain-Heart Infusion Agar can be supplemented with antibiotics, varying amounts of sodium chloride, yeast extract and serum to provide a rich medium for bacteria, yeast, and pathogenic fungi. Brain-Heart Infusion Agar (BHI Agar), is specified in many references for food and water testing. Standard Methods for the Examination of Water and Wastewater recommends Brain-Heart Infusion media in tests for the verification of fecal streptococci.
|Brain Heart Infusion (Solids)||8 g|
|Enzymatic Digest of Animal Tissue||5 g|
|Enzymatic Digest of Casein||16 g|
|Sodium Chloride||5 g|
|Disodium Phosphate||2.5 g|
Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
- Suspend 52 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
|Product #||Product Description|
|7115A||Brain-Heart Infusion Agar, 500 g|
|7115T||Brain-Heart Infusion Agar, 5 kg|
|7115C||Brain-Heart Infusion Agar, 10 kg|