Product number: 7115

Brain-Heart Infusion Agar is used for the cultivation of a wide variety of fastidious organisms in a laboratory setting. Brain-Heart Infusion Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Rosenow prepared a rich medium for culturing streptococci by combining dextrose broth and brain tissue. Hayden modified the original formula while working with dental pathogens. The current formula is a modification of Rosenow and Hayden, using dehydrated infusions of pork brain and pork heart tissue.

Brain-Heart Infusion Agar can be supplemented with antibiotics, varying amounts of sodium chloride, yeast extract and serum to provide a rich medium for bacteria, yeast, and pathogenic fungi. Brain-Heart Infusion Agar (BHI Agar), is specified in many references for food and water testing. Standard Methods for the Examination of Water and Wastewater recommends Brain-Heart Infusion media in tests for the verification of fecal streptococci.


Formula Liter
Brain Heart Infusion (Solids) 8 g
Enzymatic Digest of Animal Tissue 5 g
Enzymatic Digest of Casein 16 g
Dextrose 2 g
Sodium Chloride 5 g
Disodium Phosphate 2.5 g
Agar 13.5 g

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 52 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7115A Brain-Heart Infusion Agar, 500 g

Reference Material

7115 - Brain-Heart Infusion Agar, Product Information Sheet [English] [Portuguese]
7115 - Brain-Heart Infusion Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]

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