Product number: 7249

Bile Esculin Agar is used for the selective isolation and differentiation of group D streptococci in a laboratory setting. Bile Esculin Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Bile Esculin Agar is based on the formulation described by Swan and further evaluated by Facklam and Moody. Rochaix first noted the value of esculin hydrolysis in the identification of enterococcci. Meyer and Schonfeld added bile to the esculin medium and demonstrated 61 of 62 enterococci strains were able to grow and hydrolyze esculin, while other streptococci could not.

Molecular taxonomic studies of the genus Streptococcus have placed enterococci, previously described as group D streptococci, in the genus Enterococcus. The ability to hydrolyze esculin in the presence of bile is a characteristic of enterococci and group D streptococci. Swan compared the use of an esculin medium containing 40% bile salts with the Lancefield serological method of grouping, and reported that a positive reaction on the bile esculin medium correlated with a serological group D precipitin reaction. Facklam and Moody found that the bile esculin test provided a reliable means of identifying group D streptococci and differentiating them from non-group D streptococci.

Bile Esculin Agar is in standard procedures for the microbiological examination of food products.


Formula Liter
Beef Exract 11 g
Enzymatic Digest of Gelatin 34.5 g
Esculin 1 g
Oxbile 2 g
Ferric Ammonium Citrate 0.5 g
Agar 15 g

Final pH: 6.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 64 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7249A Bile Esculin Agar, 500 g
7249T Bile Esculin Agar, 5 kg
7249C Bile Esculin Agar, 10 kg

Reference Material

NCM0117 - Bile Esculin Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0117 - Bile Esculin Agar, Technical Specifications Sheet [English]
7249 - Bile Esculin Agar, Product Information Sheet [English] [Portuguese]

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