Product number: 7442
Bacillus cereus Agar Base is used with Polymyxin B and Egg Yolk Emulsion for the isolation and presumptive identification of Bacillus cereus in a laboratory setting. Bacillus cereus Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.
Holbrook and Anderson described a highly selective and diagnostic medium (PEMBA) for the isolation and determination of Bacillus cereus from food. The medium is formulated to detect small numbers of B. cereus in the presence of large number of contaminants. This medium differentiates B. cereus from other bacteria based on resistance to polymyxin, lack of mannitol fermentation, and presence of lecithinase. B. cereus can be found on vegetables, processed foods, and in nature. B. cereus causes gastrointestinal illness if the organism is allowed to proliferate. Outbreaks of foodborne illness have been associated with boiled and cooked rice, cooked meats, and cooked vegetables.
|Enzymatic Digest of Casein||1 g|
|Sodium Chloride||2 g|
|Magnesium Sulfate||0.1 g|
|Disodium Phosphate||2.5 g|
|Monopotassium Phosphate||0.25 g|
|Bromthymol Blue||0.10 g|
|Sodium Pyruvate||10 g|
Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Egg Yolk Emulsion, 100 mL
Polymyxin B (50,000 IU), 5 mL
- Suspend 41 g of the medium in 950 mL of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
- Cool to 45 – 50°C. Aseptically add 50 mL of Egg Yolk Emulsion (# 7982) and 10 mL of Polymyxin B (# 7997, 2 x 5 mL vials).
|Product #||Product Description|
|7442A||Bacillus cereus Agar Base, 500 g|
|7442T||Bacillus cereus Agar Base, 5 kg|
|7442C||Bacillus cereus Agar Base, 10 kg|
|7982||Egg Yolk Emulsion, 100 mL|
|7997||Polymyxin B (50,000 IU), 5 mL|
7442 - Bacillus cereus Agar Base, Product Information Sheet [English] [Portuguese]
7442 - Bacillus cereus Agar Base, Safety Data Sheet [English] [Portuguese]
7997 - Polymyxin B Supplement, 5 mL, Safety Data Sheet [English]