Product number: 7742

Acutone PPLO Broth without CV (Non-animal) is an animal-origin free formulation used for the cultivation of Mycoplasma spp. in a laboratory setting. Acutone PPLO Broth without CV (Non-animal) is not intended for use in the diagnosis of disease or other conditions in humans.

Acutone PPLO Broth without CV (Non-animal) was developed for the cultivation of Mycoplasma spp. as an alternative to PPLO Broth without CV, and is completely free of animal-origin components.

PPLO Broth without CV is prepared according to the formula described by Morton and Lecce. Acutone PPLO Broth without CV (Crystal Violet) (Non-animal) is a modification of this formula. Crystal Violet may inhibit the growth of Mycoplasma, and therefore was removed from an earlier formula described by Morton, Smith, Williams, and Eikenberg. Mycoplasma was discovered in a case of pleuropneumonia in a cow, and referred to as "pleuropneumonia-like organism" or PPLO. Mycoplasmas belong to the class of Mollicutes "soft skin", which are the smallest free-living organisms. They are pleomorphic, varying in size from 0.2 to 0.3 micromillimeters.

 

Formula Liter
Vegetable Infusion (Dehydrated) 12.5 g
Yeast Extract 3.5 g
Sodium Chloride 5 g

Final pH: 7.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement

Sterile Serum, 300 mL

Directions

  1. Dissolve 21 g of the medium in 700 mL of purified water.
  2. Heat with frequent agitation and boil to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 – 50°C and aseptically add 300 mL of the appropriate sterile serum supplement.
  5. Mix thoroughly.

Order Details

Product # Product Description
7742A Acutone PPLO Broth without CV (Non-animal), 500 g
7742T Acutone PPLO Broth without CV (Non-animal), 5 kg
7742C Acutone PPLO Broth without CV (Non-animal), 10 kg

Reference Material

7742 - Acutone PPLO Broth without CV (Non-animal), Product Information Sheet [English]
7742 - Acutone PPLO Broth without CV (Non-animal), Safety Data Sheet [English]

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