NEO-GRID® disposable units and ISO-GRID® membranes are filtration systems used on food, beverage and water samples for the detection, confirmation and enumeration of microorganisms including yeast and mould, total plate counts, coliforms, generic E. coli, E. coli O157:H7, Salmonella, Listeria, Listeria monocytogenes, Staphylococcus and more.

ISO-GRID/NEO-GRID tests are based on the concept of hydrophobic grid membrane filtration. The filter membrane is embossed with hydrophobic ink to produce a grid containing 1600 individual squares. The ink’s hydrophobic nature contains the growth of an organism isolated during the filtration process within the square of capture.

Samples are first diluted in a sterile buffer. The ISO-GRID /NEO-GRID filtration unit then is placed onto a manifold connected to a vacuum source, and a sterile filter membrane is placed in the ISO-GRID filtration unit (NEO-GRID already contains a sterile filter membrane). Using the vacuum source, a portion of the diluted sample is filtered through the filter membrane.

The membrane is transferred to an agar plate prepared with a medium formulated to promote/differentiate the growth of the target organism. Following incubation, the plate’s squares containing presumptively positive colonies are counted to yield a most probable number (MPN) result.

Approvals

    ISO-GRID/NEO-GRID Method Approvals

    Yeast and Mould Count AOAC No. 995.21
    Total Bacterial Count AOAC No. 986.32
    Total Coliform/E. coli Count AOAC No. 990.11
    Salmonella Detection AOAC No. 991.12
    E. coli O157:H7 Count AOAC No. 997.11

    Canadian Compendium of Analytical Methods-Health Canada

    Enumeration of coliforms in foods by the hydrophobic grid membrane filter method. MFHPB-17
    Enumeration of E. coli in foods by the hydrophobic grid membrane filter method. MFHPB-26
    Enumeration of fecal coliforms in foods by the hydrophobic grid membrane filter method. MFLP-55
    Determination of aerobic colony count in foods by the hydrophobic grid membrane filter method. MFLP-56
    Screening bottled water for the presence of indicator and pathogenic bacteria. MFLP-60

    Canadian Standard Methods for the Examination of Water and Wastewater

    Standard total coliform membrane filter procedure 9222B
    Fecal coliform membrane filter procedure 9222D

Results

Order Details

Product # Product Description
6802 ISO-GRID Membrane Filters, standard 1600-grid with a pore size of 0.45 µm (100 per box)
6848 NEO-GRID Test System – Contains 48 individually packaged membrane filtration systems
6866 NEO-GRID-36 Test System – Contains 48 individually packaged 36-grid disposable membrane filtration systems with a pore size of 0.45 µm

Reference Materials

Documents

6802 - Membrane Filters, ISO-GRID, 1600-Grid, Material Safety Data Sheet [English]
6848 - NEO-GRID Test System, 1600-Grid, Material Safety Data Sheet [English]
6850E - Enzyme, Papain, Material Safety Data Sheet [English]
6851E - Enzyme, APUG, Material Safety Data Sheet [English]
6853E - Cellulase Enzyme, Material Safety Data Sheet [English]
6854E - Enzyme, Hemicellulase, Material Safety Data Sheet [English]
6866 - NEO-GRID Test System, 36-Grid, Material Safety Data Sheet [English]
6901A - Media, EF-18 Agar, Material Safety Data Sheet [English]
6902A - Media, LMG Agar, Material Safety Data Sheet [English]
6903A - Media, BMA Agar, Material Safety Data Sheet [English]
6904A - Media, YM-11 Agar, Safety Data Sheet [English]
6905A - Media, TSAF Agar, Material Safety Data Sheet [English]
6907A - Media, SD-39 Agar, Safety Data Sheet [English]
6910A - Media, SCCRAM Broth, Safety Data Sheet [English]