NeoSeek™ is a breakthrough pathogen detection and identification service that provides next day, DNA-specific STEC test results for seven pathogenic Shiga toxin-producing E. coli strains (STECs). A similar service for Salmonella identification is also available.

Features of NeoSeek include:

  • STEC confirmation using DNA-specific test results.
  • 24-hour turnaround time.
  • No colony isolation required.
  • ISO 17025 accredited.

Currently, Neogen® offers next-day results from enriched samples through our GeneSeek® laboratory facilities for seven E. coli strains — O26, O45, O103, O111, O121, O145, and O157.

The NeoSeek technology uses PCR coupled with mass spectrometry-based multiplexing to develop a genetic profile for bacteria in a food sample, and then compares those results with the known genetic makeup of the reference E. coli strains to identify and differentiate the target strains. NeoSeek assays more than 86 specific genetic markers to provide faster results than conventional culture methods.

Order Details

Product # Product Description
9287 STEC Shipping Kit — shipping-ready kit for the submission of up to 15 samples for NeoSeek confirmation
9288 NeoSeek Sampling Kit — contains vials for the collection and submission of up to 15 samples for NeoSeek confirmation

Reference Materials

BIFSCO 2013 – Comparison of non-O157 Shiga Toxin-producing E. coli Detection Systems Mick Bosilevac, USDA, ARS, US Meat Animal Research Center https://www.bifsco.org/CMDocs/BIFSCO2/13_research_update.pdf

Prevalence of Enterohemorrhagic Escherichia coli O26, O45, O103, O111, O121, O145, and O157 on Hides and Preintervention Carcass Surfaces of Feedlot Cattle at Harvest. Stromberg ZR1, Baumann NW2, Lewis GL1, Sevart NJ2, Cernicchiaro N3, Renter DG3, Marx DB4, Phebus RK2, Moxley RA1. http://www.ncbi.nlm.nih.gov/pubmed/26125496

Chopyk J, Moore RM, DiSpirito Z, Stromberg ZR, Lewis GL, Renter DG, Cernicchiaro N, Moxley RA, Wommack KE. Presence of pathogenic Escherichia coli is correlated with bacterial community diversity and composition on pre-harvest cattle hides. Microbiome. 2016 Mar 22;4:9. doi: 10.1186/s40168-016-0155-4. PubMed PMID: 27000779; PubMed Central PMCID: PMC4802634. https://www.ncbi.nlm.nih.gov/pubmed/27000779

Stromberg ZR, Lewis GL, Aly SS, Lehenbauer TW, Bosilevac JM, Cernicchiaro N, Moxley RA. Prevalence and Level of Enterohemorrhagic Escherichia coli in Culled Dairy Cows at Harvest. J Food Prot. 2016 Mar;79(3):421-31. doi: 10.4315/0362-028X.JFP-15-368. PubMed PMID: 26939652. https://www.ncbi.nlm.nih.gov/pubmed/26939652

Summary Tables of No-Objection Letters Issued by FSIS for Non-O157 STEC Test Methods http://www.fsis.usda.gov/wps/portal/fsis/topics/regulatory-compliance/New-Technologies/summary-table-of-nols-non-O157-stec-test-methods/NOL-non-O157-STEC-+test-methods

 

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