16S Metagenomics - Technology
Using the DNA used to construct the 16S subunit, we are able to identify the genus and the genus species in a sample. We use a metagenomic approach with the V4 16S region. This provides a genus and/or a genus species identification for every single organism. The metagenomics approach allows companies to send in non-enriched samples, finished products, or various raw materials.
- Leveraging a proprietary 16S database, we are able to identify the genus of each bacteria present in a sample.
- Identify likely candidates for organism of concern through operational taxonomic units (OTUs).
- Show Relative abundance of the genus of bacteria present in each sample.
Using 16S for microbiome applications allows for incredible resolution in food spoilage or plate microbiome applications. 16S metagenomic analysis allows for some critical improvements over traditional subtyping methods.
Metagenomics for Food Safety and Quality Webinars
Metagenomics has many different applications in food industry. We developed a webinar series to illustrate some of the applications and differences between other next generation sequencing technologies (i.e. whole genome sequencing).
- 16S Metagenomics: A Case Study for Trouble-shooting Spoilage Sources
- Demystifying the S: WGS vs 16S
- Arming Your Scientists for Clean Label Formulations
16S vs. Traditional Methods
- Traditional methods suffer from two specific issues: volume of work, and media biases.
- The volume of work through subtyping a sample can be enormous. Each different organism needs to be isolated, and then use a traditional biochemical identification. In an environmental sample from food production, this could mean 15-20 isolates!
- The other element is viable but not culturable. Traditional subtyping methods introduce a bias by only selecting organisms that can grow in the media used in isolation. 16S circumvents this issue by obtaining amplifying and identifying the 16S sequence in a neat sample (no isolation).
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